Formulation

lyophilized (freeze-dried)

Purification

Crude extract

Biological Activity

Optimum concentration for human umbilical vein endothelial cells (HUVEC) range from 50-200 µg/ml, optimal concentration with heparin (30 µg/ml) is about 12 µg/ml.

Species Reactivity

human cells, mouse cells, rat cells, cattle cells, pig cells

Buffer

water

Reconstitution

Reconstitute the contents of the vial in 2 ml of prewarmed (37°C) sterile PBS or water. Gently rotate the vial until the contents are dissolved. This stock solution may be further diluted in sterile tissue culture media to obtain the desired working concentrations. It is recommended that medium containing diluted product is aseptically filtered prior to use.

Application Remarks

Can be used for blood endothelial cell c*tion.

Synonyms

Endothelial cell growth factor (ECGF); Endothelial cell growth supplement (ECGS)